Birthe Nielsen

Dr Birthe Nielsen MSc, PhD, AMRSC

Principal Lecturer, Analytical Science

Dr Birthe Nielsen obtained her MSc in biotechnology from the Department of Life Sciences at Aalborg University, Denmark (1998–2004) where she worked in the area of molecular plant biotechnology with Professor K.D. Grasser. After finishing with a distinction, she continued to University of Portsmouth, UK, where she undertook an industry-funded PhD (2004–07) in the biomaterials and drug delivery research group with Dr J. Tsibouklis. During this time, Dr Nielsen was assessing multifunctional polymer thin films for oral care.

In October 2007, Dr Nielsen joined the School of Science at University of Greenwich, where she has been pursuing her own research interests in mass spectrometry developing methods for the detection and quantification of salivary biomarkers of clinical and biopharmaceutical significance, and the development of inorganic/organic composite materials for hard tissue replacement.

Dr Nielsen is responsible for arranging the School of Science research seminars where external and internal speakers present their scientific findings. The seminars promote knowledge transfer and provide staff and students with an opportunity to establish new contacts.

Responsibilities within the university

  • Link tutor Pharmaceutical Sciences at MSA Cairo, Egypt.

Programme leader

  • BSc Hons Pharmaceutical Sciences

Course participation

  • Analytical and Advanced Protein Biochemistry
  • Analytical Chemistry
  • Bioinformatics
  • Instrumental Analysis
  • Pharmaceutical Analysis and Testing
  • Research and Practical Skills
  • Research Methods
  • Research projects for MSc and BSc.

Recognition

  • Associate Member of the Royal Society of Chemistry (AMRSC)

Research / Scholarly interests

Research activities involves the application of mass spectrometry for the detection of neuropeptides and steroid hormones. Over the past few years, Dr Nielsen has been working on building a deeper understanding of how specific neuropeptides ionize and behave in the gas phase.

Further, the work aims to get an understanding of factors impacting ESI and MALDI protonation in the aid to predict the mass spectrum for peptides which could be important pain and stress biomarkers for clinical research. The research includes:

  • Understanding pre-ESI factors that affect protonation
  • Understanding factors that affect ESI response (peptide characteristics such as polarity and peptide conformation)
  • H/D exchange analysis by mass spectrometry
  • Detection of steroid hormones by HPLC and LC-MS
  • Method development using nano-LC-Q-Tof MS, MALDI-TOF MS, Ion mobility MS for the detection of potential salivary biomarkers.

Dr Nielsen is also working with: Dr D. Abaye; a post doctoral researcher; master students; and PhD students.

Areas of research

  • ESI response of neuropeptides
  • Mass spectrometry (ESI and MALDI)
  • Salivary biomarkers
  • Polymer thin films for the prevention of dentine hypersensitivity, dental erosion and plaque build-up
  • Biomaterials for tissue engineering.

Dr Nielsen welcomes enquiries from potential collaborators regarding these and future projects.

Recent publications

Article

Roy, Uttam K. , Nielsen, Birthe V., Milledge, John J. (2020), Effect of post-harvest conditions on antioxidant enzyme activity in Dunaliella tertiolecta biomass. Elsevier. In: , , , . Elsevier, Biocatalysis and Agricultural Biotechnology, 27: 101661 ISSN: 1878-8181 (Print), 1878-8181 (Online) (doi: https://doi.org/10.1016/j.bcab.2020.101661) NB Item availability restricted.

Maneein, Supattra , Milledge, John J., Harvey, Patricia, Nielsen, Birthe (2020), Methane production from Sargassum muticum: effects of seasonality and of freshwater washes. Elsevier. In: , , , . Elsevier, Energy and Built Environment ISSN: 2666-1233 (Print), (doi: https://doi.org/10.1016/j.enbenv.2020.06.011).

Milledge, John J. , Nielsen, Birthe V., Maneein, Supattra, Harvey, Patricia J. (2019), A brief review of anaerobic digestion of algae for bioenergy. MDPI. In: , , , . MDPI, Energies, 12: 1166 (6) ISSN: 1996-1073 (Print), 1996-1073 (Online) (doi: https://doi.org/10.3390/en12061166).

Maneein, Supattra , Milledge, John J., Nielsen, Birthe V., Harvey, Patricia J. (2018), A review of seaweed pre-treatment methods for enhanced biofuel production by anaerobic digestion or fermentation. MDPI. In: , , , . MDPI, Fermentation, 4: 100 (4) . pp. 1-31 2311-5637 (Online) (doi: https://doi.org/10.3390/fermentation4040100).

Naclerio, Fernando , Larumbe-Zabala, Eneko, Seijo, Marcos, Ashrafi, Nadia , Nielsen, Birthe , Earnest, Conrad, P. (2018), Effects of protein vs. carbohydrate supplementation on markers of immune response in master triathletes: A randomized controlled trial. American College of Nutrition. In: , , , . American College of Nutrition, Journal of the American College of Nutrition, 38 (5) . pp. 395-404 ISSN: 0731-5724 (Print), 1541-1087 (Online) (doi: https://doi.org/10.1080/07315724.2018.1528906) NB Item availability restricted.

Mehta, Kosha J. , Seijo, Marco, Larumbe-Zabala, Eneko, Ashrafi, Nadia , Christides, Tatiana , Karsten, Bettina , Nielsen, Birthe , Naclerio, Fernando (2018), Case studies: Effects of beef, whey and carbohydrate supplementation in female master triathletes. Universidad de Alicante, Facultad de Educación. In: , , , . Universidad de Alicante, Facultad de Educación, Journal of Human Sport & Exercise, 14 (1) ISSN: 1988-5202 (Print), (doi: https://doi.org/10.14198/jhse.2019.141.14).

Milledge, John J. , Nielsen, Birthe V., Sadek, Manar S., Harvey, Patricia J. (2018), Effect of freshwater washing pretreatment on Sargassum muticum as a feedstock for biogas production. MDPI. In: , , , . MDPI, Energies, 11: 1771 (7) 1996-1073 (Online) (doi: https://doi.org/10.3390/en11071771).

Milledge, John J. , Nielsen, Birthe V., Harvey, Patricia J. (2018), The inhibition of anaerobic digestion by model phenolic compounds representative of those from Sargassum muticum. Springer Netherlands. In: , , , . Springer Netherlands, Journal of Applied Phycology, 31 (1) . pp. 779-786 ISSN: 0921-8971 (Print), 1573-5176 (Online) (doi: https://doi.org/10.1007/s10811-018-1512-4).

Roy, Uttam K. , Lavignac, Nathalie, Rahman, Azizur M., Nielsen, Birthe V. (2018), Purification of lectin and Kunitz trypsin inhibitor from soya seeds. Oxford academic. In: , , , . Oxford academic, Journal of Chromatographic Science, 56 (5) . pp. 436-442 ISSN: 0021-9665 (Print), 1945-239X (Online) (doi: https://doi.org/10.1093/chromsci/bmy018).

Ashrafi, Nadia , Lapthorn, Cristian, Pullen, Francis, Naclerio, Fernando , Nielsen, Birthe V. (2017), High performance liquid chromatography coupled to electrospray ionisation mass spectrometry method for the detection of salivary human neutrophil alpha defensins HNP1, HNP2, HNP3 and HNP4. Royal Society of Chemistry. In: , , , . Royal Society of Chemistry, Analytical Methods, 46 (9) . pp. 6482-6490 ISSN: 1759-9660 (Print), 1759-9679 (Online) (doi: https://doi.org/10.1039/C7AY01676J).

Naclerio, Fernando , Seijo, Marco, Eneko, Larumbe Zabala, Ashrafi, Nadia , Christides, Tatiana , Karsten, Bettina , Nielsen, Birthe (2017), Effects of supplementation with beef or whey protein versus carbohydrate in master triathletes. American College of Nutrition. In: , , , . American College of Nutrition, Journal of the American College of Nutrition, 36 (8) . pp. 593-601 ISSN: 0731-5724 (Print), 1541-1087 (Online) (doi: https://doi.org/10.1080/07315724.2017.1335248).

Nielsen, Birthe V. , Abaye, Daniel A., Nguyen, Minh T. L. (2017), The influence of mixed solvents volatility on charge state distribution of peptides during positive electrospray ionization mass spectrometry. Korean Society for Mass Spectrometry. In: , , , . Korean Society for Mass Spectrometry, Mass Spectrometry Letters, 8 (2) . pp. 29-33 ISSN: 2233-4203 (Print), 2093-8950 (Online) (doi: http://www.msletters.org/journal.do?method=journalintro&journalSeq=J000011).

De Santis, E. , Edwards, A. A., Alexander, Bruce, Holder, S. J. , Biesse-Martin, A.-S. , Nielsen, B. V. , Mistry, D. , Waters, L. , Siligardi, G. , Hussain, R. (2016), Selective complexation of divalent cations by a cyclic α,β-peptoid hexamer: a spectroscopic and computational study. Royal Society of Chemistry. In: , , , . Royal Society of Chemistry, Organic and Biomolecular Chemistry, 14 (48) . pp. 11371-11380 ISSN: 1477-0520 (Print), 1477-0539 (Online) (doi: https://doi.org/10.1039/C6OB01954D).

Naclerio, Fernando , Larumbe-Zabala, Eneko, Ashrafi, Nadia, Seijo Bujia, Marcos , Nielsen, Birthe , Allgrove, Judith , Earnest, Conrad P. (2016), Effects of protein–carbohydrate supplementation on immunity and resistance training outcomes: a double-blind, randomized, controlled clinical trial. Springer Verlag. In: , , , . Springer Verlag, European Journal of Applied Physiology, 117 (2) . pp. 267-277 ISSN: 1439-6319 (Print), 1439-6327 (Online) (doi: https://doi.org/10.1007/s00421-016-3520-x) NB Item availability restricted.

Milledge, John J. , Nielsen, Birthe V., Bailey, David (2016), High-value products from macroalgae: the potential uses of the invasive brown seaweed, Sargassum muticum. Springer Netherlands. In: , , , . Springer Netherlands, Reviews in Environmental Science and Bio/Technology, 15 (1) . pp. 67-88 ISSN: 1569-1705 (Print), 1572-9826 (Online) (doi: http://dx.doi.org/10.1007/s11157-015-9381-7) NB Item availability restricted.

Tanner, Amy Vivien , Nielsen, Birthe Vejby, Allgrove, Judith (2014), Salivary and plasma cortisol and testosterone responses to interval and tempo runs and a bodyweight-only circuit session in endurance-trained men. Routledge Francis & Taylor. In: , , , . Routledge Francis & Taylor, Journal of Sports Sciences, 32 (7) . pp. 680-689 ISSN: 0264-0414 (Print), 1466-447X (Online) (doi: http://dx.doi.org/10.1080/02640414.2013.850594).

Abaye, Daniel A. , Nielsen, Birthe, Boateng, Joshua S. (2013), Detection of homocysteine in human saliva using protein precipitation and analysis by liquid chromatography and electrospray ionization quadrupole time-of-flight mass spectrometry: Profiles in healthy adults. Bentham Science Publishers. In: , , , . Bentham Science Publishers, Protein and Peptide Letters, 20 (12) . pp. 1382-1389 ISSN: 0929-8665 (Print), 1875-5305 (Online) (doi: http://dx.doi.org/10.2174/092986652012131112142531).

Abaye, Daniel A. , Nielsen, Birthe, Boateng, Joshua (2013), Determination of homocysteine in human saliva by liquid chromatography and electrospray ionization quadrupole time-of-flight mass spectrometry: profiles in healthy adults. Bentham Science Publishers. In: , , , . Bentham Science Publishers, Protein and Peptide Letters, 20 (12) . pp. 1382-1389 ISSN: 0929-8665 (Print), 1875-5305 (Online) (doi: http://www.ingentaconnect.com/content/ben/ppl/2013/00000020/00000012/art00010).

Nielsen, Birthe V. and , Abaye, Daniel A. (2013), Influence of electrolytes and a supercharging reagent on charge state distribution and response of neuropeptide ions generated during positive electrospray ionisation mass spectrometry. IM Publications. In: , , , . IM Publications, European Journal of Mass Spectrometry, 19 (5) . pp. 335-344 ISSN: 1469-0667 (Print), 1751-6838 (Online) (doi: http://dx.doi.org/10.1255/ejms.1246).

Conference item

Milledge, John J. , Nielsen, Birthe, Harvey, Patricia (2018), Sargassum – Menace, Methane, Mouth and More. In: 3rd Medway Engineering Conference, 20 June 2018, University of Greenwich, Medway Campus , . , (doi: ).

Maneein, Supattra , Nielsen, Birthe, Milledge, John J. (2018), Comparison between automated anaerobic digestion test systems for determination of biochemical methane potential of cellulose. In: 5th International Conference on Renewable Energy Gas Technology, 3-4 May, Toulouse, France , . , (doi: https://regatec.org/posters/).

Milledge, John , Temukum, Ngomah Kien, Ratana, Amrita, Nielsen, Birthe , Harvey, Patricia (2015), Anaerobic Digestion (AD) of Novel Wastes. In: 3rd Faculty of Engineering & Science Research Symposium, September 2015, University of Greenwich , . , (doi: ).

Conference proceedings

Ashrafi, Nadia , Seijo, Marcos, Pullen, Frank, Nielsen, Birthe V. , Smith, Joshua , Wilkinson, Christian , Fu, Yue , Miller, Jack , Larumbe-Zabala, Eneko , Naclerio, Fernando (2015), A multi-ingredient containing, proteins, carbohydrate and creatine does not attenuate humoral immune response or performance decrease compared to carbohydrate during resistance training. International Society of Sport Nutrition. In: , , , Proceedings of the Twelfth International Society of Sports Nutrition (ISSN) Conference and Exp. International Society of Sport Nutrition, Journal of the International Society of Sports Nutrition, 12 (S1) ISSN: 1550-2783 (Print), 1550-2783 (Online) (doi: http://dx.doi.org/10.1186/1550-2783-12-S1-P34).